Kavitha, VenkatachalamVenkatachalamKavithaSnega, ViswanathanViswanathanSnegaViswanathamurthi, PeriasamyPeriasamyViswanathamurthiJebiti Haribabu2025-10-102025-10-10202510530509; 15734994https://hdl.handle.net/20.500.12740/23487A novel probe ITQ (9-(((E)-1 H-inden-1-ylidene)methyl)-8-(3-(((E)-1 H-inden-1-ylidene)methyl)phenoxy)-2,3,6,7-tetrahydro-1 H,5 H-pyrido[3,2,1ij]quinolone) was successfully designed and synthesized to detect amino acid lysine (Lys). The selective sensing behavior of the probe ITQ was observed using absorption and emission spectral results. Further, the probe ITQ exhibits a strong binding affinity for Lys [1.4 × 104 M− 1] and detects and quantifies Lys even in its nanomolar concentration. Moreover, the probe ITQ detects Lys at 1:2 binding stoichiometry with suitable biological pH [4, 5, 6, 7, 8, 9, 10–11]. Furthermore, the probe ITQ was also successfully utilized to detect Lys in tablets, real samples (avocado, soyabean and pork) and in live HeLa cells. © 2025 Elsevier B.V., All rights reserved.restrictedAccessHELA CELLSNANOMOLARREAL SAMPLESSELECTIVE SENSINGTABLETSLYSINEFLUORESCENT DYESAMINO ACIDSBINDING ENERGYLANTHANUM COMPOUNDSAMINO-ACIDSFOOD SAMPLESHELA CELLQUINOLONESSIMPLE++SYNTHESISEDTABLETPROBESFLUORESCENT DYECHEMICAL STRUCTURECHEMISTRYFOOD ANALYSISHELA CELL LINEHUMANPROCEDURESSPECTROFLUOROMETRYSYNTHESISHUMANSMOLECULAR STRUCTURESPECTROMETRY, FLUORESCENCEArtículo https://doi.org/10.1007/s10895-023-03523-6