Godoy-Cumillaf, AndresAndresGodoy-CumillafFuentes-Merino, PaolaPaolaFuentes-Merinode Souza-Lima, JosivaldoJosivaldode Souza-LimaParra-Saldias, MaribelMaribelParra-SaldiasDuclos-Bastias, DanielDanielDuclos-BastiasFarias-Valenzuela, ClaudioClaudioFarias-ValenzuelaMerellano-Navarro, EugenioEugenioMerellano-NavarroBruneau-Chavez, JoseJoseBruneau-ChavezRodriguez-Gutierrez, EvaEvaRodriguez-Gutierrez2026-07-072026-07-072026JOURNAL OF CLINICAL MEDICINE, 15(9), 3191 (2026). https://doi.org/10.3390/jcm150931912077-0383https://hdl.handle.net/20.500.12740/24673Background/Objectives: University life is often accompanied by unhealthy lifestyle behaviors, reduced physical activity, lower fitness levels, and a high prevalence of mental health symptoms. Daily step count has emerged as a practical indicator of habitual physical activity;" however, evidence on its association with cardiorespiratory fitness and symptoms of depression, anxiety, and stress in university students remains limited. Therefore, this study examined the association of daily step count with cardiorespiratory fitness and symptoms of depression, anxiety, and stress in university students. Methods: This cross-sectional association study included a convenience sample of 120 students aged 18 to 25 years from a single university. Daily step count was assessed over seven consecutive days using a Xiaomi Mi Band 9. Cardiorespiratory fitness was evaluated with the 20 m shuttle run test, and symptoms of depression, anxiety, and stress were measured using the Depression, Anxiety and Stress Scale-21 Items (DASS-21). Partial correlations, ANCOVA, MANCOVA, binary logistic regression, and restricted cubic spline models were performed after adjustment for sex, age, and socioeconomic status. Results: Higher daily step count was associated with greater cardiorespiratory fitness and with lower symptoms of depression, anxiety, and stress, although the associations with mental health symptoms were weak and not uniform across outcomes. Restricted cubic spline models showed inverse non-linear associations for mental health symptoms, with steeper inverse gradients at lower step-count levels and a tendency to level off at higher volumes, approximately around 9000 steps/day. For cardiorespiratory fitness, the association was positive across the step-count range. Step counts around 7500 steps/day were associated with lower odds of elevated symptoms of depression, anxiety, and stress. Conclusions: A higher daily step count was associated with more favorable mental health symptom profiles and greater cardiorespiratory fitness in this sample of university students."info:eu-repo/semantics/openAccessmental healthphysical fitnessphysical activitypedometerArticulohttps://doi.org/10.3390/jcm15093191