Serrano, EdisonEdisonSerranoSimpfendorfer, RobertRobertSimpfendorferMedina, AlbertoAlbertoMedinaSandoval, CarlosCarlosSandovalMartínez, AlexisAlexisMartínezMorales, RodrigoRodrigoMoralesDavies, Simon J.Simon J.Davies2026-10-092026-10-092021-09Serrano, Edison; Simpfendorfer, Robert; Medina, Alberto; Sandoval, Carlos; Martínez, Alexis; Morales, Rodrigo; Davies, Simon J. (2021-09). Partially replacing fish oil with microalgae ( Schizochytrium limacinum and Nannochloropsis oceanica ) in diets for rainbow trout ( Oncorhynchus mykiss ) reared in saltwater with reference to growth performance, muscle fatty acid composition and liver ultrastructure. Aquaculture Research, 52(9), 4401-4413. https://doi.org/10.1111/are.152791355-557X1365-2109https://hdl.handle.net/20.500.12740/25117The effect of dietary inclusion of a mixture of microalgae meal (Schizochytrium limacinum and Nannochloropsis oceanica) (1:1 ratio) on growth performance, gene expression, histology and muscle fatty acid composition of rainbow trout (Oncorhynchus mykiss) reared in saltwater was investigated. Three experimental extruded diets containing 0%, 9% and 17% of a mixture of microalgae meal were evaluated in triplicate groups during 10-week bioassay. The results showed that growth performance and feed conversion ratio were significantly reduced by increasing the dietary inclusion of a mixture of microalgae meal. No significant trends were observed with respect to feed intake and relative gene expression of hsp70, scarb1, IL-12 and myod. Conversely, increasing dietary levels of a mixture of microalgae meal led to a decrease in vacuolar degeneration of hepatocytes. A reduction of 23% on the amount of 20:5n-3 fatty acid in the muscle was found between the fish fed control and the highest level of the microalgae meal blend diets. Nevertheless, 22:6n-3 fatty acid content did not vary among dietary treatments. These results demonstrate that a mixture of microalgae meal has a potential to be included up to 90 g kg(-1) within diets for rainbow trout as a sustainable replacement for fish oil.http://purl.org/coar/access_right/c_abf2feed performancen− 3 fatty acidsNannochloropsis oceanicarainbow troutSchizochytrium limacinumArticulohttps://doi.org/10.1111/are.15279