Simón, Layla Y.Layla Y.SimónTorres, KeilaKeilaTorresGuerrero, SimonSimonGuerreroOyarzun-Ampuero, Felipe A.Felipe A.Oyarzun-AmpueroQuest, Andrew F.G.Andrew F.G.Quest2025-10-102025-10-10202507533322; 19506007https://hdl.handle.net/20.500.12740/23370Metastasis, the dissemination of cancer cells, is promoted by metabolic reprogramming and the expression of Caveolin-1 (CAV1). Curcumin and phlorotannins have antioxidant and antitumoral activities. This study compares the potential anti-metastatic effects of phloroglucinol (phlorotannin monomer, PHG) and curcumin-loaded nanoemulsions (CUR-NEM) in CAV1-expressing cells. To that end, the metastatic cancer cell lines (MDA-MB-231 and B16-F10), which either express or not CAV-1, were treated with either PHG or CUR-NEM. The results demonstrate that PHG and CUR-NEM prevent cancer cell migration in vitro and metastasis in vivo of CAV1-expressing cells. Moreover, in the cancer cells treated with PHG or CUR-NEM, a significant reduction in glycolysis due to the inhibition of hexokinase activity was detected. These findings show that both PHG and CUR-NEM prevent metastasis in a preclinical animal model and link this ability to the inhibition of hexokinase 2, highlighting their potential significance as cancer therapeutics. © 2025 Elsevier B.V., All rights reserved.openAccessBIOACTIVE NATURAL COMPOUNDSCANCER METASTASISCURCUMINGLYCOLYSIS INHIBITORSPHLOROTANNINPOLYPHENOL6 PHOSPHOFRUCTOKINASEHEXOKINASEPHLOROGLUCINOLPYRUVATE KINASEANTINEOPLASTIC AGENTSCAVEOLIN 1EMULSIONSHK2 PROTEIN, HUMANHEXOKINASE 1MONOMERANTINEOPLASTIC AGENTNANOPARTICLEANIMAL EXPERIMENTANIMAL MODELANIMAL TISSUEANTINEOPLASTIC ACTIVITYARTICLEB16-F10 CELL LINECANCER CELLCANCER INHIBITIONCELL MIGRATIONCELL VIABILITYCONTROLLED STUDYCYTOTOXIC CONCENTRATIONDRUG EFFECTDRUG MECHANISMEXPERIMENTAL METASTASISEXTRACELLULAR ACIDIFICATION RATEGLYCOLYSISHUMANHUMAN CELLIC50IN VITRO STUDYLUNG METASTASISMDA-MB-231 CELL LINEMETABOLIC REPROGRAMMINGMETASTASISMETASTASIS INHIBITIONMETASTASIS POTENTIALMOLECULAR DOCKINGNANOEMULSIONNONHUMANPROTEIN EXPRESSIONPROTEIN EXPRESSION LEVELPROTEIN PHOSPHORYLATIONREDUCTION (CHEMISTRY)WESTERN BLOTTINGANIMALC57BL MOUSECELL MOTIONCHEMISTRYEMULSIONFEMALEMETABOLISMMOUSETUMOR CELL LINEANIMALSCELL LINE, TUMORCELL MOVEMENTHUMANSMICEMICE, INBRED C57BLNANOPARTICLESNEOPLASM METASTASISArtículo https://doi.org/10.1016/j.biopha.2025.118339