Here, we report the draft genome sequence and assembly of the Penicillium sp. strain E22, which was isolated from Antarctic soil of Deception Island, South Shetland Islands close to the Antarctic Peninsula. The genome was sequenced using a 2 # 250 bp paired-end method by Illumina MiSeq 60 0 0. The genome assembly was performed using softwares implemented in the Kbase web service. The phylogenetic tree of strain E22 comparing its internal transcribed spacer (ITS) region with the other Penicillium showed high genetic similarity to Penicillium griseofulvum MN545450 and Penicillium camemberti MT530220. Draf genome of Penicillium sp. strain E22 comprises 33,653 coding sequences, with a high G + C content of 48.32% and a total size of 37,484,944 bp. This C1 [Peng, Teoh Chui; Wong, Clemente M. V. L.] Univ Malaysia Sabah, Biotechnol Res Inst, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia. [Lavin, Paris; Fierro-Vasquez, Natalia] Univ Antofagasta, Fac Ciencias Mar & Recursos Biol, Dept Biotecnol, Antofagasta 1240300, Chile. [Lavin, Paris] Univ Antofagasta, Ctr Invest Inmunol & Biotecnol Biomed Antofagasta, Antofagasta 1240300, Chile. [Pedraza, Romulo Oses] Univ Atacama, Ctr Reg Invest & Desarrollo Sustentable Atacama CR, Copiapo, Chile. [Purcarea, Cristina] Romanian Acad, Inst Biol Bucharest, 296 Splaiul Independentei, Bucharest 060031, Romania. [Yong, Sheau Ting] Univ Oxford, INEOS Oxford Inst Antimicrobial Res, Dept Biol, Oxford, England. C3 Universiti Malaysia Sabah; Universidad de Antofagasta; Universidad de Antofagasta; Universidad de Atacama; Romanian Academy; Institute of Biology Bucharest; University of Oxford