The genus Anchorius Casey, 1900 (Coleoptera: Biphyllidae) is reported for the first time in Chile. Based on the study of specimens from collections, Anchorius dollyae sp. nov. is described and illustrated. This new taxon is distinguished from other known Anchorius species by its distinctive distribution pattern of rows of pronotal setae, the presence of well-defined femoral lines and lateral lines of the first ventrite, incomplete metaventral discrimen, and different aedeagal morphology compared to morphologically similar species. This new species represents the southernmost record of this genus in South America. Finally, this finding provides new Anchorius habitat associations, including temperate and Mediterranean forests ecosystems in Chile. C1 [Tello, Francisco] Univ Atacama, Fac Ciencias Nat, Dept Quim & Biol, Copiapo, Chile. [Tello, Francisco] Univ Austral Chile, Lab Salud Bosques, Inst Conservac Biodivers & Terr ICByT, Valdivia, Chile. [Tello-Arriagada, Cristobal] Univ Austral Chile, Escuela Pregrad Licenciatura Ciencias con Menc, Valdivia, Chile. C3 Universidad de Atacama; Universidad Austral de Chile; Universidad Austral de Chile