An anthracene thiosemicarbazone with a pyrrolidine substitution (APyTSC) was synthesized from 9-anthracenecarboxaldehyde and N(4)-pyrrolidine thiosemicarbazide in ethanol, using glacial acetic acid as the catalyst. APyTSC was initially confirmed using NMR, FT-IR, and HRMS spectroscopic techniques. Single-crystal XRD analysis further revealed that the compound has been formed, classified as orthorhombic with space group Pca21. The synthesized compound was evaluated for its potential application in latent fingerprint detection. APyTSC powder demonstrated strong adherence to fingerprint ridges without leaving background staining. Its vibrant yellow color provided high contrast on both dark and select light surfaces, enhancing visibility. Compared to conventional black and white fingerprint powders, APyTSC yielded significantly clearer prints across a variety of surfaces. These results highlight its promising utility in forensic investigations, particularly in scenarios requiring rapid and high-contrast fingerprint development.